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7.What is your reagent composition

 

Standard calibration substance with preservatives

Standard diluent 1.5ml0.01M PBS

HRP-Conjugate reagent 6mlHRP-linked Avidin

Sample diluent 6ml1% BSACAS # 9048-46-8, 0.05%Tween-20 PBS

Chromogen Solution A  6ml32μL30% H2O2(CAS# 7722-84-1)

Chromogen Solution B  6ml0.5 ml  2 mg/mL TMBCAS # 54827-17-7

Stop Solution 6ml   2mol/L H2SO4(CAS# 7664-93-9)

wash  solution 20ml 30X  30X TBS

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