Summary:

 

Purpose

Our Quick Step Rat High Density Lipoprotein (HDL)ELISA Kit is to assay HDL levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HDL. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HDL is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HDL and HRP conjugated HDL antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HDL. You can calculate the concentration of HDL in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0346Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0	0.047	0.0440
		0.041
S1	36ng/ml	1.967	1.9610
		1.955
S2	24ng/ml	1.315	1.3200
		1.325
S3	12ng/ml	0.685	0.6930
		0.701
S4	6ng/ml	0.391	0.3880
		0.385
S5	3ng/ml	0.183	0.1875
		0.192

 

 

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