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1. Application
This kit is suitable for the preparatory treatment of residual DNA detection in biological product samples. Through chemical lysis and adsorption of magnetic beads, it can extract ultra-low amount of DNA from various sample types to the maximum extent.
This kit is recommended for sample preparation for host cell DNA residual(qPCR-based)detection kits,including:
CHO Host Cell Dna Residue Detection kit
HEK293 Host Cell Dna Residue Detection kit
NS0 Host Cell Dna Residue Detection kit
Vero Host Cell Dna Residue Detection kit
E.Coli Host Cell Dna Residue Detection kit
Pichia Pastoris Host Cell Dna Residue Detection kit
2. Product Components and Storage
After receiving the kit, please check the contents according to the table provided. If the quantity is correct, storage them under the corresponding conditions.
This kit provides reagents sufficient for analysis of 100 samples.
Components
Quantity
Storage
1
Lysis Buffer
10mL/Vial*1
Room Temperature
2
Wash Solution A
25mL/Vial*1
Add 25mL Anhydrous Ethanol in the first use
3
Wash Solution B
15mL/Vial*1
Add 45mL Anhydrous Ethanol in the first use
4
Elution Buffer
10mL/Vial*1
5
Magnetic Bead Suspension
1mL/Tube*2
4℃
6
Proteinase K[1]
1mL/Tube*2
-20℃
7
Glycogen
0.8mL/Tube*1
8
POLY(A) Potassium Salt
0.5mL/Tube*1
Note:[1]To prevent any potential impact on its effect caused by repeated freeze-thaw cycles, it is recommended to aliquot the component upon first use.
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