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Human 5-Nucleotidase(5-NT)  ELISA Kit
Human 5-Nucleotidase(5-NT) ELISA Kit
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  • NO.:SL0031Hu
    Sample Volume:10ul
    Wavelength:450nm
    Assay range:0.8 ng/ml -50 ng/ml
    Sensitivity:0.1 ng/ml
    Standard:72 ng/ml
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Drug Names

Generic NameHuman 5-Nucleotidase(5-NT) ELISA Kit

Purpose

Our Human 5-Nucleotidase(5-NT) ELISA kit is to assay 5-NT levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 5-NT. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 5-NT is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 5-NT and HRP conjugated 5-NT antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 5-NT. You can calculate the concentration of 5-NT in the samples by comparing the OD of the samples to the standard curve.

 

 

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