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Fish beta catenin (beta-Cat)  ELISA Kit

Fish beta catenin (beta-Cat) ELISA Kit

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  • Cat NO.:SL0083FI
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:3 pg/ml - 200 pg/ml
  • Sensitivity:0.6 pg/ml
  • Qualitative:
  • Standard:270 pg/ml
  • Price:$528
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameFish beta catenin (beta-Cat) ELISA Kit

Purpose

Our Fish beta catenin (beta-Cat) ELISA kit is to assay beta-Cat levels in Fish serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to beta-Cat. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for beta-Cat is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain beta-Cat and HRP conjugated beta-Cat antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of beta-Cat. You can calculate the concentration of beta-Cat in the samples by comparing the OD of the samples to the standard curve.

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