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Rat Ferroportin-1 (FPN-1) ELISA Kit

Rat Ferroportin-1 (FPN-1) ELISA Kit

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  • Cat NO.:SL1577Ra
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:45 pg/ml - 4000 pg/ml
  • Sensitivity:12 pg/ml
  • Standard:5400 pg/ml
  • Price:$498
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameRat Ferroportin-1 (FPN-1)ELISA Kit

Purpose

Our Rat Ferroportin-1 (FPN-1)ELISA kit is to assay Ferroportin-1 (FPN-1) levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to FPN-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for FPN-1 added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain FPN-1 and HRP conjugated FPN-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of FPN-1. You can calculate the concentration of FPN-1 in the samples by comparing the OD of the samples to the standard curve.

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