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Immunomodulatory Effects of Fucosylated Chondroitin Sulfate from Stichopus Chloronotus on Raw 264.7 Cells
SUNLONG BIOTECH / 2024-01-09
    • Type: RAW 264.7 / cell-culture-supernatants

    • Author:Jiang, S., Yin, H., Qi, X., Song, W., Shi, W., Mou, J. & Yang, J.

    • Periodical:Carbohydrate polymers 251, 117088 (2021)

    • Article source

    Sea cucumbers were nutritional food and traditional Chinese medicine. In this study, fucosylated chondroitin sulfate from sea cucumber Stichopus chloronotus (fCS-Sc), a potential anticoagulant agent and immunological adjuvant, was investigated for its immune activation effects on RAW 264.7 macrophage for the first time. The results indicated that fCS-Sc could significantly promote the proliferation, the pinocytic activity of RAW 264.7 cells, and the production of NO, TNF-α, IL-1β, and IL-6. The fluorescence labeling assay indicated that fCS-Sc could bind to the macrophage. Moreover, the specific pattern recognition receptor inhibition assays showed that toll-like receptor 4 (TLR4) and TLR2 were involved in the recognition of fCS-Sc. Western blot assays indicated that fCS-Sc could induce degradation of cytoplasm IκB-α, and promotion of NF-κB p65 subunit translocation to nucleus, leading to a functional improvement of macrophage through NF-κB pathway. The results suggested that fCS-Sc might served as a promising candidate of immunomodulator.

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