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Plant-Derived Phosphocholine Facilitates Cellular Uptake of Anti-Pulmonary Fibrotic Hjt-Srna-M7
SUNLONG BIOTECH / 2024-01-09
  • Author:Du, J., Liang, Z., Xu, J., Zhao, Y., Li, X., Zhang, Y., Zhao, D., Chen, R., Liu, Y., Joshi, T., Chang, J., Wang, Z., Zhang, Y., Zhu, J., Liu, Q., Xu, D. & Jiang, C.

  • Periodical:Science China. Life sciences 62, 309-320 (2019)

  • Article source

Pulmonary fibrosis, a progressive chronic disease with a high mortality rate, has limited treatment options. Currently, lung transplantation remains the only effective treatment. Here we report that a small RNA, HJT-sRNA-m7, from a Chinese herbal medicine Hong Jing Tian (HJT, RHODIOHAE CRENULATAE RADIX ET RHIZOMA, Rhodiola crenulata) can effectively reduce the expressions of fibrotic hallmark genes and proteins both in alveolar in vitro and in mouse lung tissues in vivo. We also discovered over one hundred oil-soluble chemicals from HJT decoctions, most of which are found in lipid extracts from other Chinese herbals decoctions, including Pu Gong Ying (PGY, TARAXACI HERBA, Taraxacum mongolicum), Chuan Xin Lian (CXL, changed to "ANDROGRAPHIS HERBA, Andrographis paniculata"), and Jin Yin Hua (JYH, lonicera japonica or Honeysuckle). We identified the active component in these decoctions as two forms of phosphocholines, PC (18:0/18:2) and PC (16:0/18:2). These PCs potentially could form liposomes with small RNAs to enter human alveolar and gastric cells. Our experimental results suggest an unprecendent lipid complex route through which botanic small RNA can enter human bodies. Our results provide an innovative treatment strategy for oral delivery of siRNAs as therapeutic medication.

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