How to draw standard curve of elisa kit?
Sunlong Biotech / 2023-10-23
Quadratic Polynomial Fit!
Normally people draw ELISA standard curve by using specialized tools for curve fitting, such as: Curve Expert 1.4
Below is "Curve Exert1.4" software, for example, draw the ELISA standard curve as follows:
2. X-axis: input the OD values of standard, Y-axis: input values corresponding to the concentration.
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3.Starting from "1", click the curve names in the upper right corner one by one, and select the curve with the best fitting degree (the closer the "r" value is to 1, the better the fitting degree).
4 Right-click on the curve graph, and click [information] to obtain the formula and parameters.
5.Right-click on the curve graph, select Analyze, enter the OD value, and click Calculate to calculate the content
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