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Standard :calibration substance with preservatives
Standard diluent 1.5ml:0.01M PBS
HRP-Conjugate reagent 6ml:HRP-linked Avidin
Sample diluent 6ml:1% BSA(CAS # 9048-46-8), 0.05%Tween-20 PBS
Chromogen Solution A 6ml:32μL30% H2O2(CAS# 7722-84-1)
Chromogen Solution B 6ml:0.5 ml 2 mg/mL TMB(CAS # 54827-17-7)
Stop Solution 6ml :2mol/L H2SO4(CAS# 7664-93-9)
wash solution 20ml 30X : 30X TBS
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