TEL: +86 571 56623320    EMAIL: [email protected]

Quantitative Analysis and Pharmacokinetics Study of Integrin Antagonist Ap25 in Rat Plasma
SUNLONG BIOTECH / 2024-01-09
  • Author:Li, Y., Xu, H., Hu, J., Zhang, F., Liu, Z., Zhang, C., Yang, Y., Pu, C., Huang, L. & Wen, X.

  • Periodical:Protein and peptide letters 20, 584-590 (2013)

  • Article source

BALB/c mice were immunized by highly immunogenic recombinant proteins containing amino acid sequence of integrin antagonist AP25. Antibody against AP25 was prepared and purified by affinity chromatography. An indirect enzyme-linked immunosorbent assay for qualitative analysis of AP25 in rat plasma samples was successfully established. The assay was successfully applied to determine the pharmacokinetic parameters of AP25 in SD rats by intravenous administration of AP25 and then the rat plasma protein binding of AP25 were determined in vitro. In order to investigate the specificity of ELISA for detection of prototype AP25 in plasma, the proportion of AP25 prototype drug in the ELISA signal value was validated by HPLC. These results can serve as valuable future clinical trials.

User Comment(Total0User Comment Num)

  • No comment
Total 0 records, divided into1 pages First Prev Next Last
Username: Anonymous user
E-mail:
Rank:
Content:
Verification code: captcha

Call us

+86 571 56623320

Address

Room 1-315, Kongle Changqing Building, No. 160 Guangye Road,Gongshu District, Hangzhou City, Zhejiang Province, China

Join Us with

Leave a message
* To protect against spam, please pass the CAPTCHA test below.