Aliases:	ACTR2; Actin Related Protein 2; ARP2; ARP2 Actin Related Protein 2 Homolog; Actin-Related Protein 2; Actin-Like Protein 2; ARP2 (Actin-Related Protein 2, Yeast) Homolog; ARP2 Actin-Related Protein 2 Homolog (Yeast)
Background:	UniProt Entry: P61160; NCBI Gene Entry: 10097
Application Information	Molecular Weight: Predicted, 45 kDa, observed, 37 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB610 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Arp2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Arp2 expression in C2C12 cells using Arp2 antibody (Cat#66172, 1:2,000). Green, isotype control; red, Arp2.

Immunocytochemical staining of C2C12 cells with Arp2 antibody (Cat#66172, 1:1,000). Nuclei were stained blue with DAPI; Arp2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Arp2 antibody (Cat#66172). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Arp2 antibody (Cat#66172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-Arp2 antibody (Cat#66172). Arp2 expression in wild type (WT) and Arp2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Arp2 antibody (Cat#66172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

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