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Product Name Anti-phospho-HDAC8 (Ser39)/Cy7 Chinese Name Cy7标记的磷酸化组蛋白去乙酰化酶8抗体 Alias HDAC8 (Phospho-Ser39); HDAC8 (Phospho S39); HD 8; HD8; HDAC 8; HDACL 1; HDACL1; Histone deacetylase 8; Histone deacetylase like 1; RPD 3; RPD3; CDA07; Hdac8; HDAC8_HUMAN. Product Type Phosphorylated anti Research Area Tumour Cardiovascular Cell biology immunology Developmental biology Chromatin and nuclear signals Signal transduction Stem cells Apoptosis transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, ) Applications
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 42kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human HDAC8 around the phosphorylation site of Ser39 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Function:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
Subunit:
Interacts with PEPB2-MYH11, a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11 produced by the inversion Inv(16)(p13q22), a translocation associated with acute myeloid leukemia of M4EO subtype. The PEPB2-MYH1 fusion protein also interacts with RUNX1, a well known transcriptional regulator, suggesting that the interaction with HDAC8 may participate in the conversion of RUNX1 into a constitutive transcriptional repressor. Interacts with CBFA2T3. Interacts with phosphorylated SMG5/EST1B; this interaction protects SMG5 from ubiquitin-mediated degradation. Associates with alpha-SMA (smooth muscle alpha-actin).
Subcellular Location:
Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
Tissue Specificity:
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
Post-translational modifications:
Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
Similarity:
Belongs to the histone deacetylase family. HD type 1 subfamily.
Database links:Entrez Gene: 55869 Human
Entrez Gene: 70315 Mouse
Omim: 300269 Human
SwissProt: Q9BY41 Human
SwissProt: Q8VH37 Mouse
Unigene: 310536 Human
Unigene: 328128 Mouse
Unigene: 208476 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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