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ACTIVITY TEST
Buffer Formulation 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Traits Freeze-dried powder Purity > 97% Isoelectric Point 8.6 Applications Cell culture; Activity Assays.
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Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1β can induced IL-8 production in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x106 cells/mL, and allowed to attach overnight before treated with certain concentrations (0.1ng/mL-1000ng/mL) of IL-1β for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1β have shown in Figure1.
Figure. IL-8 levels in the cell supernatant of A549 induced by IL-1β.USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Figure. Gene Sequencing (extract)
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Figure. SDS-PAGE
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Sample: Recombinant IL1b, Mouse;
Antibody: Rabbit Anti-Mouse IL1b Ab (PAA563Mu02)
Figure. Western Blot
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