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Source Prokaryotic expression Host E.coli Endotoxin Level <1.0EU per 1µg (determined by the LAL method) Subcellular Location Secreted, Cytoplasm, Endoplasmic reticulum lumen, Extracellular matrix Predicted Molecular Mass 21.3kDa Accurate Molecular Mass 21kDa(Analysis of differences refer to the manual) Residues & Tags Glu18~Ser193 with N-terminal His Tag Buffer Formulation PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Traits Freeze-dried powder Purity > 97% Isoelectric Point 5.9 Applications Positive Control; Immunogen; SDS-PAGE; WB.
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Image
Figure. SDS-PAGE
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