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Source Eukaryotic expression Host 293F cell Endotoxin Level <1.0EU per 1µg (determined by the LAL method) Subcellular Location Secreted Predicted Molecular Mass 17.8kDa Accurate Molecular Mass 16kDa(Analysis of differences refer to the manual) Residues & Tags Gln28~Glu172 with N-terminal His Tag Buffer Formulation 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Traits Freeze-dried powder Purity > 95% Isoelectric Point 8.7 Applications Positive Control; Immunogen; SDS-PAGE; WB.
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Image
Figure. SDS-PAGE
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