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Source Prokaryotic expression Host E.coli Endotoxin Level <1.0EU per 1µg (determined by the LAL method) Subcellular Location Lysosome, Cytoplasm, Golgi apparatus, Endoplasmic reticulum lumen, Endosome Predicted Molecular Mass 40.7kDa Accurate Molecular Mass 41kDa(Analysis of differences refer to the manual) Residues & Tags Ser1529~Val1853 with N-terminal His Tag Buffer Formulation 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Traits Freeze-dried powder Purity > 97% Isoelectric Point 8.3 Applications Positive Control; Immunogen; SDS-PAGE; WB.
SEQUENCE
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USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Image
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Figure. SDS-PAGE
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