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Source Prokaryotic expression Host E.coli Endotoxin Level <1.0EU per 1µg (determined by the LAL method) Subcellular Location Membrane, Cytoplasm Predicted Molecular Mass 34.5kDa Accurate Molecular Mass 42kDa(Analysis of differences refer to the manual) Residues & Tags Met1~Gly179 with Two N-terminal Tags, His-tag and SUMO-tag Buffer Formulation PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Traits Freeze-dried powder Purity > 90% Isoelectric Point 8.7 Applications Positive Control; Immunogen; SDS-PAGE; WB.
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Image
Figure. SDS-PAGE
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