Source	Prokaryotic expression
Host	E.coli
Endotoxin Level	<1.0EU per 1µg (determined by the LAL method)
Subcellular Location	Secreted, Lysosome, Cytoplasm, Golgi apparatus, Endoplasmic reticulum lumen, Endosome
Predicted Molecular Mass	58.1kDa
Accurate Molecular Mass	58kDa(Analysis of differences refer to the manual)
Residues & Tags	Trp164~Phe428 with N-terminal His and GST Tag
Buffer Formulation	20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits	Freeze-dried powder
Purity	> 90%
Isoelectric Point	5.9
Applications	Positive Control; Immunogen; SDS-PAGE; WB.

 

SEQUENCE

 

USAGE

 

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

 

STORAGE

 

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

 

STABILITY

 

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Image


SDS-PAGE Image

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