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Source | Prokaryotic expression |
Host | E.coli |
Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
Subcellular Location | Nucleus, Mitochondrion, Cytoplasm |
Predicted Molecular Mass | 30.6kDa |
Accurate Molecular Mass | 31kDa(Analysis of differences refer to the manual) |
Residues & Tags | Tyr27~Phe311 with N-terminal His Tag |
Buffer Formulation | 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Traits | Freeze-dried powder |
Purity | > 97% |
Isoelectric Point | 6.4 |
Applications | Positive Control; Immunogen; SDS-PAGE; WB. |
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Source Prokaryotic expression Host E.coli Endotoxin Level <1.0EU per 1µg (determined by the LAL method) Subcellular Location Nucleus, Mitochondrion, Cytoplasm Predicted Molecular Mass 30.6kDa Accurate Molecular Mass 31kDa(Analysis of differences refer to the manual) Residues & Tags Tyr27~Phe311 with N-terminal His Tag Buffer Formulation 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Traits Freeze-dried powder Purity > 97% Isoelectric Point 6.4 Applications Positive Control; Immunogen; SDS-PAGE; WB.
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Image
Figure. SDS-PAGE
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