Drug Names

Generic Name: Human Glomerular Basement Membrane Antibody  (GBM-Ab) ELISA Kit

Purpose

Our Human Glomerular Basement Membrane Antibody (GBM-Ab) ELISA Kit is to for the qualitative determination of GBM-Ab in Human serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an antigen specific to GBM-Ab,  make it to solid-phase antigen. Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for GBM is added to each Microplate well and incubated, so the antigen-antibody-Enzyme labeled antigen complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain GBM-Ab and HRP conjugated GBM antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of GBM-Ab is determined by comparing with the CUTOFF value.

 

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