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Sunlong Medical™ Human TIMP-1 ELISA Kit
Sunlong Medical™ Human TIMP-1 ELISA Kit
TIMP1 elisa kit | TIMP metallopeptidase inhibitor 1 elisa kit | CLGI | collagenase inhibitor | EPA | EPO | erythroid potentiating activity | erythroid-potentiating activity | fibroblast collagenase inhibitor | FLJ90373 | HCI | metalloproteinase inhibitor
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  • NO.:EL0229Hu
    Species:Human
    Assay range:31.25pg/mL-2000pg/mL
    Sensitivity:3.94 pg/mL
    Sample Volume:10 μL
    Assay type:Sandwich Method
    Validity:Two Years
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Details

Cat NO.:
EL0229Hu
Product:
Sunlong Medical™ Human TIMP-1 ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
90% - 105%
Mean Spike Recovery:
0.94
CV of Intra plate:
4.9%-5.4%
CV of Inter plate:
6.4% - 7.3%
NCBI_Gene:
7076
UniProtKB:
P01033

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TIMP-1A monoclonal antibody
Human TIMP-1A freeze-dried standard
TIMP-1A detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TIMP-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TIMP-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TIMP-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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