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Sunlong Medical™ Human LDL Receptor/LDLR ELISA Kit
Sunlong Medical™ Human LDL Receptor/LDLR ELISA Kit
LDLR elisa kit | low density lipoprotein receptor elisa kit | FH | FHC | FHCL1 | LDL receptor | LDLCQ2 | low-density lipoprotein receptor | low-density lipoprotein receptor class A domain-containing protein 3
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  • NO.:EL0179Hu
    Species:Human
    Assay range:125.00pg/mL-8000pg/mL
    Sensitivity:6.32 pg/mL
    Sample Volume:10 μL
    Assay type:Sandwich Method
    Validity:Two Years
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Details

Cat NO.:
EL0179Hu
Product:
Sunlong Medical™ Human LDL Receptor/LDLR ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
101%112%
Mean Spike Recovery:
1.07
CV of Intra plate:
3.0% - 4.3%
CV of Inter plate:
2.3% - 3.6%
NCBI_Gene:
3949
UniProtKB:
P01130

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-LDLR monoclonal antibody
Human LDLR freeze-dried standard
LDLR detect Antibody
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human LDLR antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, LDLR present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of LDLR in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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