Cat NO.:	EL0154Hu
Product:	Sunlong Medical™ Human Apolipoprotein E/Apo E ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	85% - 117%
Mean Spike Recovery:	1.08
CV of Intra plate:	3.5% - 5.0%
CV of Inter plate:	3.8% - 5.0%
NCBI_Gene:	348
UniProtKB:	P02649

 

SAMPLE VOLUME	90 μL 1×Assay Buffer and 10 μL prediluted sample
SENSITIVITY	1.71 pg/mL
RANGE	78.13 pg/mL – 5000 pg/mL
ASSAY TIME	3.5 h
RECOVERY	85% – 117%
AVERAGE RECOVERY	1.08
INTRA PRECISION	3.5% – 5.0%
INTER-PRECISION	3.8% – 5.0%
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein E/Apo E monoclonal antibody Human Apolipoprotein E/Apo E freeze-dried standard Apolipoprotein E/Apo E detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo E antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo E present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo E in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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