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Sunlong Medical™ Human IL-10 ELISA Kit
Sunlong Medical™ Human IL-10 ELISA Kit
IL10 elisa kit | interleukin 10 elisa kit | CSIF | cytokine synthesis inhibitory factor | GVHDS | IL-10 | IL10A | interleukin-10 | MGC126450 | MGC126451 | T-cell growth inhibitory factor | TGIF
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Regular members: $455.2
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  • NO.:EL0271Hu
    Species:Human
    Assay range:3.13pg/mL-200pg/mL
    Sensitivity:0.43 pg/mL
    Sample Volume:Serum | plasma;50 μL;Cell culture supernatant;100 μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:226
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Cat NO.:
EL0271Hu
Product:
Sunlong Medical™ Human IL-10 ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
80% - 117%
Mean Spike Recovery:
0.91
CV of Intra plate:
3.2 % - 5.8 %
CV of Inter plate:
3.0 % - 8.3 %
NCBI_Gene:
3586
UniProtKB:
P22301

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-10 monoclonal antibody
Human IL-10 freeze-dried standard
IL-10 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-10 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-10 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 
















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He***22024-09-11
Ga***12024-08-31
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Ga***22024-07-17
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