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Sunlong Medical™ Human IL-13 ELISA Kit
Sunlong Medical™ Human IL-13 ELISA Kit
IL13 elisa kit | interleukin 13 elisa kit | allergic rhinitis | ALRH | BHR1 | Bronchial hyperresponsiveness-1 bronchial asthma | IL-13 | interleukin-13 | MGC116786 | MGC116788 | MGC116789 | P600
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Regular members: $455.2
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Cat NO.:
EL0237Hu
Product:
Sunlong Medical™ Human IL-13 ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
82% - 110%
Mean Spike Recovery:
0.98
CV of Intra plate:
1.7 % - 2.0 %
CV of Inter plate:
2.2 % - 4.1 %
NCBI_Gene:
3596
UniProtKB:
P35225

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-13 monoclonal antibody
Human IL-13 freeze-dried standard
IL-13 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-13 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-13 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-13 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

 

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