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Sunlong Medical™ Human uPAR ELISA Kit
Sunlong Medical™ Human uPAR ELISA Kit
PLAUR elisa kit | plasminogen activator elisa kit | urokinase receptor elisa kit | CD87 | monocyte activation antigen Mo3 | U-PAR | u-plasminogen activator receptor form 2 | UPAR | URKR | urokinase plasminogen activator surface receptor | urokinase-type p
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Regular members: $455.2
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Details

Cat NO.:
EL0197Hu
Product:
Sunlong Medical™ Human uPAR ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
87% - 114%
Mean Spike Recovery:
1.01
CV of Intra plate:
3.0 % - 4.7 %
CV of Inter plate:
2.1 % - 5.0 %
NCBI_Gene:
5329
UniProtKB:
Q03405
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-uPAR monoclonal antibody
Human uPAR freeze-dried standard
uPAR detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human uPAR antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, uPAR present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of uPAR in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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Be***22024-08-17
Za***12024-04-12
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