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Sunlong Medical™ Human?Prostate-Specific Antigen/PSA ELISA Kit
Sunlong Medical™ Human?Prostate-Specific Antigen/PSA ELISA Kit
KLK3 elisa kit | kallikrein related peptidase 3 elisa kit | APS | gamma-seminoprotein | hK3 | kallikrein 3 | prostate specific antigen | kallikrein-3 | kallikrein-related peptidase 3 | KLK2A1 | P-30 antigen | prostate specific antigen | prostate-specifi
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Details

Cat NO.:
EL0159Hu
Product:
Sunlong Medical™ Human?Prostate-Specific Antigen/PSA ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
100% - 118%
Mean Spike Recovery:
1.09
CV of Intra plate:
3.9 % - 4.5 %
CV of Inter plate:
4.1 % - 4.5 %
NCBI_Gene:
354
UniProtKB:
P07288

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PSA monoclonal antibody
Human PSA freeze-dried standard
PSA detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PSA antibodies are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate for incubation. After washing to remove unbound substances, the detection antibody labeled with horseradish peroxidase is added for further incubation. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of PSA in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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