Cat NO.:	EL0092Hu
Product:	Sunlong Medical™ hCG ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	95% - 119%
Mean Spike Recovery:	1.08
CV of Intra plate:	3.9% - 4.7%
CV of Inter plate:	3.8% - 4.5%
NCBI_Gene:	Inquiry
UniProtKB:	Inquiry

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-hCG monoclonal antibody Human hCG freeze-dried standard hCG detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-hCG monoclonal antibodies are pre-coated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, the hCG present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, horseradish peroxidase (HRP)-labeled streptavidin is added. After washing, the TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of hCG in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

 

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