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Sunlong Medical™ Human TSH ELISA Kit
Sunlong Medical™ Human TSH ELISA Kit
Thyroid Stimulating Hormone ELISA Kit
Total
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Regular members: $455.2
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Details

Cat NO.:
EL0089Hu
Product:
Sunlong Medical™ Human TSH ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
97% - 118%
Mean Spike Recovery:
1.06
CV of Intra plate:
2.5% - 4.2%
CV of Inter plate:
3.6% - 5.1%
NCBI_Gene:
Inquiry
UniProtKB:
Inquiry
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TSH monoclonal antibody
Human TSH freeze-dried standard
Human TSH detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TSH antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the TSH present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TSH in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

 

Partial purchase records(bought amounts latest20)

UsernameQuantitybought time
Ia***22024-09-08
Ni***32024-09-08
Ia***12024-08-26
Le***32024-07-30
Ni***22024-07-30
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