Cat NO.:	EL0079Mo
Product:	Sunlong Medical™ Mouse ST2/IL-33R ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	83% - 116%
Mean Spike Recovery:	1.02
CV of Intra plate:	3.9 % - 4.8 %
CV of Inter plate:	3.6 % - 4.9 %
NCBI_Gene:	17082
UniProtKB:	P14719

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ST2/IL-33R monoclonal antibody Mouse ST2/IL-33R freeze-dried standard ST2/IL-33R detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific monoclonal antibodies against mouse IL-16 are precoated on a high-affinity ELISA plate. Standard samples, test samples, and HRP-labeled detection antibodies are added to the wells of the ELISA plate. After incubation, the IL-16 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of IL-16 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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