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Cat NO.:EL0070Mo Product:Sunlong Medical™ Mouse IL-12/IL-23p40 ELISA Kit Storage4℃ (unopened) for two years Shipping Condition4℃ Sample Type:Serum | plasma | cell culture supernatant and other biological samples Spike Recovery Range:85% - 112% Mean Spike Recovery:0.98 CV of Intra plate:2.3 % - 3.0 % CV of Inter plate:3.6 % - 6.2 % NCBI_Gene:16160 UniProtKB:P43432
PLATFORM ELISA PLATE Detachable 96-well plate SIZE 96T/48T STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. DELIVERY 4℃ blue ice transportation COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-12/IL-23p40 monoclonal antibody
Mouse IL-12/IL-23p40 freeze-dried standard
IL-12/IL-23p40 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing FilmASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-12/IL-23p40 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-12/IL-23p40 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-12/IL-23p40 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
7.3Frontiers in Immunology 2023-06-234.026AAPS PHARMSCITECH 2023-01-254.493Journal of Immunology Research 2023-03-275.6Journal of Functional Foods 2023-09-165.195JOURNAL OF ETHNOPHARMACOLOGY 2023-01-108.2INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2023-09-1615.304BIOMATERIALS 2023-03-3114.5Acta Pharmaceutica Sinica B 2023-06-2010Advanced Healthcare Materials 2023-06-21
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