Cat NO.:	EL0064Mo
Product:	Sunlong Medical™ Mouse CCL20/MIP-3α ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	82% - 96%
Mean Spike Recovery:	0.9
CV of Intra plate:	4.5 % - 7.5 %
CV of Inter plate:	5.6 % - 6.9 %
NCBI_Gene:	20297
UniProtKB:	O89093

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL20/MIP-3α monoclonal antibody Mouse CCL20/MIP-3α freeze-dried standard CCL20/MIP-3α detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Monoclonal antibodies specific for mouse CCL20 are pre-coated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, the CCL20 present in the sample binds to the solid-phase antibody and the detection antibody. After washing to remove unbound substances, streptavidin-HRP is added. After washing again, the TMB chromogenic substrate is added and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of CCL20 in the sample. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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