Cat NO.:	EL0038Ra
Product:	Sunlong Medical™ Rat IL-2 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	85% - 110%
Mean Spike Recovery:	1.02
CV of Intra plate:	3.5 % - 6.8 %
CV of Inter plate:	5.5 % - 8.2 %
NCBI_Gene:	116562
UniProtKB:	P17108

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-2 monoclonal antibody Rat IL-2 freeze-dried standard IL-2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-2 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

1. Electrospun polymer fibers modified with FK506 for the long-term treatment of acute cardiac allograft rejection in a heart transplantation model

Biomaterials Science 2023-05-02

 

2. Microneedle-mediated transdermal delivery of FK506 for treatment of acute cardiac allograft rejection

MATERIALS & DESIGN 2023-10-18

 

3. Paeoniflorin inhibits hepatocellular carcinoma growth by reducing PD-L1 expression

BIOMEDICINE & PHARMACOTHERAPY 2023-08-17

 

4. An injectable signal-amplifying device elicits a specific immune response against malignant glioblastoma

Acta Pharmaceutica Sinica B 2023-06-20

 

5. An Experimental Study

JOURNAL OF ULTRASOUND IN MEDICINE 2023-03-14

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