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Cat NO.:EL0134Hu Product:Sunlong Medical™ Human C-peptide ELISA Kit Storage4℃ (unopened) for two years Shipping Condition4℃ Sample Type:Serum | plasma | cell culture supernatant and other biological samples Spike Recovery Range:75% - 123% Mean Spike Recovery:0.95 CV of Intra plate:3.8 % - 7.4 % CV of Inter plate:3.6% - 4.5% NCBI_Gene:4880 UniProtKB:P23582
PLATFORM ELISA PLATE Detachable 96-well plate SIZE 96T/48T STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. DELIVERY 4℃ blue ice transportation COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-C-peptide monoclonal antibody
Human C-peptide freeze-dried standard
C-peptide detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing FilmASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human C-Peptide antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, C-Peptide present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of C-Peptide in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
Username | Quantity | bought time |
Ka*** | 1 | 2024-08-15 |
Is*** | 1 | 2024-08-04 |
Fr*** | 3 | 2024-07-02 |
Ow*** | 1 | 2024-05-15 |
Ha*** | 1 | 2024-03-21 |
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