Cat NO.:	EL0124Hu
Product:	Sunlong Medical™ Human B7-H1/PD-L1/CD274 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	81% - 104%
Mean Spike Recovery:	0.85
CV of Intra plate:	3.4% - 5.8%
CV of Inter plate:	1.3% - 4.9%
NCBI_Gene:	29126
UniProtKB:	Q9NZQ7

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-B7-H1 monoclonal antibody Human B7-H1 freeze-dried standard B7-H1 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human B7-H1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, B7-H1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of B7-H1 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

  

 

 Homeobox D9 drives the malignant phenotypes and enhances the Programmed death ligand-1 expression in non-small cell lung cancer cells via binding to Angiopoietin-2 promoter

World Journal of Surgical Oncology 2023-03-13

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