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Sunlong Medical™ Human GRO-β/CXCL2 ELISA Kit
Sunlong Medical™ Human GRO-β/CXCL2 ELISA Kit
CXCL2 elisa kit | C-X-C motif chemokine ligand 2 elisa kit | C-X-C motif chemokine 2 | chemokine C-X-C motif ligand 2 | CINC-2a | gro-beta | GRO2 | GRO2 oncogene | GROb | growth-regulated protein beta | macrophage inflammatory protein 2-alpha | melanoma
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  • NO.:EL0120Hu
    Species:Human
    Assay range:4.69pg/mL-300pg/mL
    Sensitivity:0.53 pg/mL
    Sample Volume:10 μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:114
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Cat NO.:
EL0120Hu
Product:
Sunlong Medical™ Human GRO-β/CXCL2 ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
85% - 110%
Mean Spike Recovery:
99%
CV of Intra plate:
4.8 % - 5.0 %
CV of Inter plate:
3.8 % - 4.7 %
NCBI_Gene:
2920
UniProtKB:
P19875
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-GRO-β monoclonal antibody
Human GRO-β freeze-dried standard
GRO-β detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human GRO-β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, GRO-β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of GRO-β in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

  

 

 
 
 

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