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Sunlong Medical™ Human IFN-α ELISA Kit
Sunlong Medical™ Human IFN-α ELISA Kit
IFNA1 elisa kit | interferon alpha 1 elisa kit | IFL | IFN | IFN-ALPHA | IFN-alpha 1b | IFN-alpha-1/13 | IFN-alphaD | IFNA@ | IFNA13 | interferon alpha 1b | interferon alpha-1/13 | interferon alpha-D | interferon-alpha1 | interferon | alpha 1 | leIF D | M
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  • NO.:EL0031Hu
    Species:Human
    Assay range:15.63pg/mL-1000pg/mL
    Sensitivity:1.04 pg/mL
    Sample Volume:Serum | plasma;50 μL;Cell culture supernatant;100 μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:219
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Cat NO.:
EL0031Hu
Product:
Sunlong Medical™ Human IFN-α ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
97% - 118%
Mean Spike Recovery:
1.06
CV of Intra plate:
4.5 % - 5.3 %
CV of Inter plate:
3.9 % - 6.5 %
NCBI_Gene:
3439
UniProtKB:
P01562

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IFN-α monoclonal antibody
Human IFN-α freeze-dried standard
IFN-α detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IFN-α antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, IFN-α present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IFN-α in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

 1. The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1

JOURNAL OF IMMUNOLOGY 2023-08-09

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Xa***32024-07-26
Ta***12024-03-18
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