Cat NO.:	EL0018Mo
Product:	Sunlong Medical™ Mouse sICAM-1/CD54 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	89% - 112%
Mean Spike Recovery:	1.02
CV of Intra plate:	3.6 % - 4.0 %
CV of Inter plate:	4.1 % - 5.1 %
NCBI_Gene:	15894
UniProtKB:	P13597

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sICAM-1/CD54 monoclonal antibody Mouse sICAM-1/CD54 freeze-dried standard sICAM-1/CD54 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse sICAM-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sICAM-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sICAM-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

1. Protective effect of phosphorylated Athyrium multidentatum (Doll.) Ching polysaccharide on vascular endothelial cells in vitro and in vivo

Chemical Biology & Drug Design 2023-08-07

 

2. Lactoferrin ameliorated obesity-induced endothelial dysfunction by inhibiting the Tak1/IL-18/eNOS pathway between PVAT and vascular endothelium

FREE RADICAL BIOLOGY AND MEDICINE 2023-12-28

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