Cat NO.:	EL0017Ra
Product:	Sunlong Medical™ Rat IgM ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	80% - 110%
Mean Spike Recovery:	0.98
CV of Intra plate:	4.8 % - 7.7 %
CV of Inter plate:	6.1 % - 7.1 %
NCBI_Gene:	Inquiry
UniProtKB:	Inquiry

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IgM monoclonal antibody Rat IgM freeze-dried standard IgM detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IgM antibodies are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, IgM present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, detection antibodies labeled with horseradish peroxidase (HRP) are added and incubated. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IgM in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

1. Accumulative Effects of Multifrequency Microwave Exposure with 1.5 GHz and 2.8 GHz on the Structures and Functions of the Immune System

International Journal of Environmental Research and Public Health 2023-03-12

 

2. Ginsenoside Rg3 endows liposomes with prolonged blood circulation and reduced accelerated blood clearance

JOURNAL OF CONTROLLED RELEASE 2023-10-25

 

3. Myricetin ameliorates experimental autoimmune myocarditis in mice by modulating immune response and inhibiting MCP-1 expression

EUROPEAN JOURNAL OF PHARMACOLOGY 2023-01-25

 

4. Sleep deprivation reduced LPS-induced IgG2b production by up-regulating BMAL1 and CLOCK expression

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2023-11-25

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