Cat NO.:	EL0013Hu
Product:	Sunlong Medical™ Human Her3/ErbB3 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	82% - 102%
Mean Spike Recovery:	0.97
CV of Intra plate:	3.2 % - 4.6 %
CV of Inter plate:	2.0 % - 4.9 %
NCBI_Gene:	2065
UniProtKB:	P21860

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
ASSAY PRINCIPLE	This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-human ErbB3 antibodies are pre-coated on a high-affinity ELISA plate. Standard samples, test samples, and detection antibodies labeled with horseradish peroxidase (HRP) are added to the wells of the ELISA plate. After incubation, the ErbB3 present in the sample binds to the solid-phase antibody and the detection antibody. After washing, the TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of ErbB3 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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