Cat NO.:	EL0001Hu
Product:	Sunlong Medical™ Human FGFR2 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	87% - 119%
Mean Spike Recovery:	1.07
CV of Intra plate:	3.0 % - 7.6 %
CV of Inter plate:	5.1 % - 6.8 %
NCBI_Gene:	2263
UniProtKB:	P21802

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with mouse anti-human FGFR2 capture antibody Human FGFR2 freeze-dried standard Streptavidin-HRP标记Human FGFR2单克隆 detect Antibody Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film Standard Diluent
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human FGFR2 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the FGFR2 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, detection antibodies labeled with horseradish peroxidase (HRP) are added and incubated. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of FGFR2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

 

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