Cat NO.:	EL0120Mo
Product:	Sunlong Medical™ Mouse IGFBP-5 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Spike Recovery Range:	93 %-110 %
Mean Spike Recovery:	1
CV of Intra plate:	2.65% - 4.80%
CV of Inter plate:	3.79% - 5.30%
NCBI_Gene:	Inquiry
UniProtKB:	Inquiry

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
ASSAY PRINCIPLE	This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-mouse IGFBP-5 antibodies are precoated on a high-affinity ELISA plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the plate and incubated, allowing the IGFBP-5 in the samples to bind to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, a chromogenic substrate TMB is added for color development under light protection. The intensity of the color reaction is directly proportional to the concentration of IGFBP-5 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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Totalgoods,subtotals:￥Checkout