Cat NO.:	EL0080Hu
Product:	Sunlong Medical™ Human Endothelin 1/ET-1 Competitive ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	90% - 108%
Mean Spike Recovery:	0.98
CV of Intra plate:	3.1% - 6.2%
CV of Inter plate:	4.2%-7.0%
NCBI_Gene:	1906
UniProtKB:	P05305

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ET-1 monoclonal antibody Human ET-1 freeze-dried standard Angiotensinogen/AGT/ SerpinA8 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes a solid-phase competitive enzyme-linked immunosorbent assay (ELISA) technique. Specific monoclonal antibodies against human ET-1 are precoated on a high-affinity ELISA plate. Biotinylated ET-1 and unlabeled ET-1 or samples are added into the wells of the ELISA plate, competing to bind to the limited binding sites on the coated antibody. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is inversely proportional to the concentration of ET-1 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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