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Cat NO.:EL0012Hu Product:Sunlong Medical™ Human MICA ELISA Kit Storage4℃ (unopened) for two years Shipping Condition4℃ Sample Type:Serum | plasma | cell culture supernatant and other biological samples Spike Recovery Range:80% - 120% Mean Spike Recovery:0.96 CV of Intra plate:4.6% - 8.4% CV of Inter plate:4.8% - 9.3% NCBI_Gene:100507436 UniProtKB:Q29983
PLATFORM ELISA PLATE Detachable 96-well plate SIZE 96T/48T STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. DELIVERY 4℃ blue ice transportation COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with mouse anti-human MICA capture antibody
Human MICA freeze-dried standard
Human MICA detect Antibody
Streptavidin-HRP
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing FilmASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MICA antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MICA present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of MICA in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
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