Cat NO.:	EL0006Mt
Product:	Sunlong Medical™ Cortisol Competitive ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	80% - 130%
Mean Spike Recovery:	1.18
CV of Intra plate:	4.3% - 4.6%
CV of Inter plate:	6.4% - 7.8%
NCBI_Gene:	Inquiry
UniProtKB:	Inquiry

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Competitive monoclonal antibody Cortisol competitive freeze-dried standard Competitive detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes a competitive enzyme-linked immunosorbent assay (ELISA) technique. Rabbit anti-mouse antibody is precoated on a high-affinity ELISA plate. Cortisol-specific monoclonal antibodies are added to the wells of the plate and incubated, allowing them to bind to the solid-phase antibodies. After washing, HRP-labeled cortisol and unlabeled cortisol or samples are added, competing for limited binding sites on the monoclonal antibody. After washing to remove unbound substances, a chromogenic substrate is added, and the intensity of the color reaction is inversely proportional to the concentration of cortisol in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). This assay kit has been validated for human, mouse, and rat serum samples, but cortisol from other species is also expected to be detectable.

1. Mental health, sleep quality, and hormonal circadian rhythms in pregnant women with threatened preterm labor: a prospective observational study

BMC Pregnancy and Childbirth 2023-07-07

 

2. Plasma biomarkers and delirium in critically ill patients after cardiac surgery: A prospective observational cohort study

HEART & LUNG 2023-02-17

 

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