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Sunlong Medical™ Mouse TRANCE/TNFSF11/RANKL ELISA Kit
Sunlong Medical™ Mouse TRANCE/TNFSF11/RANKL ELISA Kit
TNFSF11 elisa kit | TNF superfamily member 11 elisa kit | Ly109l | ODF | OPGL | osteoclast differentiation factor | RANKL | TNF-related activation-induced cytokine | Trance
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  • NO.:EL0067Mo
    Species:Mouse
    Assay range:31.25 pg/mL - 2000 pg/mL
    Sensitivity:4.23 pg/mL
    Sample Volume:20μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:138
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Cat NO.:
EL0067Mo
Product:
Sunlong Medical™ Mouse TRANCE/TNFSF11/RANKL ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
83% - 116%
Mean Spike Recovery:
0.94
CV of Intra plate:
2.9 % - 7.9 %
CV of Inter plate:
3.1 % - 5.4 %
NCBI_Gene:
21943

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TRANCE monoclonal antibody
Mouse TRANCE freeze-dried standard
TRANCE detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse TRANCE antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TRANCE present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of TRANCE in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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