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Drug Names
Generic Name: Human Tumor necrosis factor receptor type 1 associated DEATH
domain protein (TRADD) ELISA Kit
Purpose
Our Human Tumor necrosis factor receptor type 1 associated DEATH domain protein (TRADD) ELISA kit is to assay TRADD levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TRADD. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for TRADD is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TRADD and HRP conjugated TRADD antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TRADD. You can calculate the concentration of TRADD in the samples by comparing the OD of the samples to the standard curve.
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