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Drug Names
Generic Name: Rat 12-hydroxyeicosatetraenoic acid (12-HETE) ELISA Kit
Purpose
Our Rat 12-hydroxyeicosatetraenoic acid (12-HETE) ELISA kit is to assay 12-HETE levels in Rat serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 12-HETE. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for 12-HETE is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 12-HETE and HRP conjugated 12-HETE antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 12-HETE. You can calculate the concentration of 12-HETE in the samples by comparing the OD of the samples to the standard curve.
Username | Quantity | bought time |
Mo*** | 1 | 2024-08-19 |
Ja*** | 1 | 2024-06-22 |
Is*** | 2 | 2024-04-02 |
Ja*** | 2 | 2022-10-02 |
Um*** | 3 | 2021-08-15 |
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