Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Chlamydia trachomatis belongs to the family Chlamydiaceae and is an obligate intracellular parasitic prokaryotic organism. Gram-negative, first isolated in 1955 and officially named in 1973. Chlamydia trachomatis is widely distributed around the world, and its host is limited to humans. In most cases, there are no symptoms after infection. Without treatment, it can cause trachoma, genitourinary tract infections and lymphogranuloma venereum. Currently, there are 20 known serotypes, with different serotypes Cause different diseases. Serotypes A, B, Ba and C only infect the ocular mucosa and cause conjunctivitis . These serotypes are rarely isolated from the genitourinary tract; serotypes D, Da, E, F, G, H, I, Ia, J, Ja and K can infect the mucosal tissues of the genitourinary tract and eyes, causing urethritis, epididymitis, pelvic inflammatory disease, endometritis, vaginitis, ectopic pregnancy, fallopian tube infertility, proctitis, conjunctiva inflammatory disease, neonatal pneumonia, etc.; serotypes L1, L2, L2a, L2b and L3 are invasive and can infect lymph nodes through mucosal tissue and cause lymphogranuloma venereum. Chlamydia trachomatis is the leading cause of blindness and the most common sexually transmitted bacterium, infecting an estimated 500 million people worldwide every year. Chlamydia trachomatis can be transmitted sexually, through daily necessities, equipment, etc. It can also be transmitted by swimming. Mothers and infants can be transmitted through birth canal contact infection, intrauterine infection and puerperal infection.

 

Chlamydia trachomatis can be detected through in vitro culture, with good specificity but sensitivity Low, the test is more complex. Serological methods can use direct fluorescent antibody detection, enzyme-labeled immunoreaction, complement fixation test, ELISA, agglutination test, etc. PCR is an in vitro enzymatic method for synthesizing specific DNA fragments. Its sensitivity and specificity are better than other methods. The detection speed is fast and can be completed in only two to three hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.

 

This kit is designed for a specific endonuclease gene sequenceThe primers and probes specifically identify all serotypes of Chlamydia trachomatis and have been verified by BLAST to have no cross-reactivity with other biological genomes. This kit detected 11 types of bacteria or viruses with similar distribution ranges, and no non-specific signals were found. 274 samples were tested and 70 positives were obtained, which is more sensitive than the immunofluorescence method . This kit is suitable for the detection and identification of Chlamydia trachomatis.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

沙眼衣原体（Chlamydia trachomatis）属于衣原体科（Chlamydiaceae），是一种专性细胞内寄生的原核生物，革兰氏阴性，于1955年首次分离，1973年正式命名。沙眼衣原体在全球范围内广泛分布，宿主限于人类，多数情况下感染后没有症状，不经治疗可引起沙眼、泌尿生殖道感染和性病淋巴肉芽肿，目前已知有20种血清型，不同血清型引起不同的疾病。血清型A、B、Ba和C只感染眼粘膜，引起结膜炎（沙眼），这些血清型很少能从泌尿生殖道分离得到；血清型D、Da、E、F、G、H、I、Ia、J、Ja和K可感染泌尿生殖道和眼部的粘膜组织，引起尿道炎、附睾炎、盆腔炎症、子宫内膜炎、阴道炎、异位妊娠、输卵管性不孕、直肠炎、结膜炎，以及新生儿肺炎等；血清型L1、L2、L2a、L2b和L3则具有侵入性，可穿过粘膜组织感染淋巴结，引起性病淋巴肉芽肿。沙眼衣原体是最主要的致盲性因素，也是最常见的性传播细菌，全球每年估计感染人数约为5亿人。沙眼衣原体可通过性传播，也可通过日常用品、器械等传播，游泳也可传播，母婴可以通过产道接触感染、宫内感染及产褥期感染进行传播。

沙眼衣原体可通过体外培养进行检测，特异性好，但敏感性低，试验较复杂。血清学方法可以采用直接荧光抗体检测、酶标免疫反应、补体结合试验、ELISA、凝集试验等。PCR是一种体外酶促合成特异性DNA 片段的方法，灵敏度和特异性均好于其他方法，检测速度快，只需两三个小时即可完成。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒针对一个特异性的核酸内切酶基因序列设计引物和探针，特异性识别沙眼衣原体所有血清型，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了11种分布范围类似的细菌或病毒，未发现非特异性信号；对274个样本进行检测，获得70个阳性，灵敏度高于免疫荧光法（61个阳性）。本试剂盒适用于沙眼衣原体的检测和鉴定。

 

 

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